Dynamic real-time imaging of living cell traction force by piezo-phototronic light nano-antenna array.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34039600.
- Also identified by DOI 10.1126/sciadv.abe7738 and PMC identifier 8153726.
- Licence recorded as CC BY-NC.
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Abstract
Dynamic mapping of the cell-generated force of cardiomyocytes will help provide an intrinsic understanding of the heart. However, a real-time, dynamic, and high-resolution mapping of the force distribution across a single living cell remains a challenge. Here, we established a force mapping method based on a "light nano-antenna" array with the use of piezo-phototronic effect. A spatial resolution of 800 nm and a temporal resolution of 333 ms have been demonstrated for force mapping. The dynamic mapping of cell force of live cardiomyocytes was directly derived by locating the antennas' positions and quantifying the light intensities of the piezo-phototronic light nano-antenna array. This study presents a rapid and ultrahigh-resolution methodology for the fundamental study of cardiomyocyte behavior at the cell or subcellular level. It can provide valuable information about disease detection, drug screening, and tissue engineering for heart-related studies.