Nanofluidics for Simultaneous Size and Charge Profiling of Extracellular Vesicles.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34061534.
- Also identified by DOI 10.1021/acs.nanolett.0c02558.
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Abstract
Extracellular vesicles (EVs) are cell-derived membrane structures that circulate in body fluids and show considerable potential for noninvasive diagnosis. EVs possess surface chemistries and encapsulated molecular cargo that reflect the physiological state of cells from which they originate, including the presence of disease. In order to fully harness the diagnostic potential of EVs, there is a critical need for technologies that can profile large EV populations without sacrificing single EV level detail by averaging over multiple EVs. Here we use a nanofluidic device with tunable confinement to trap EVs in a free-energy landscape that modulates vesicle dynamics in a manner dependent on EV size and charge. As proof-of-principle, we perform size and charge profiling of a population of EVs extracted from human glioblastoma astrocytoma (U373) and normal human astrocytoma (NHA) cell lines.
Medical subject headings
- Extracellular Vesicles
- Glioblastoma