Repression by the <i>Arabidopsis</i> TOPLESS corepressor requires association with the core mediator complex.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34075876.
- Also identified by DOI 10.7554/eLife.66739 and PMC identifier 8203292.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The plant corepressor TOPLESS (TPL) is recruited to a large number of loci that are selectively induced in response to developmental or environmental cues, yet the mechanisms by which it inhibits expression in the absence of these stimuli are poorly understood. Previously, we had used the N-terminus of <i>Arabidopsis thaliana</i> TPL to enable repression of a synthetic auxin response circuit in <i>Saccharomyces cerevisiae</i> (yeast). Here, we leveraged the yeast system to interrogate the relationship between TPL structure and function, specifically scanning for repression domains. We identified a potent repression domain in Helix 8 located within the CRA domain, which directly interacted with the Mediator middle module subunits Med21 and Med10. Interactions between TPL and Mediator were required to fully repress transcription in both yeast and plants. In contrast, we found that multimer formation, a conserved feature of many corepressors, had minimal influence on the repression strength of TPL.
Medical subject headings
- Arabidopsis
- Arabidopsis/genetics
- Arabidopsis/metabolism
- Arabidopsis Proteins
- Arabidopsis Proteins/chemistry
- Arabidopsis Proteins/genetics
- Arabidopsis Proteins/metabolism
- Co-Repressor Proteins
- Co-Repressor Proteins/chemistry
- Co-Repressor Proteins/genetics
- Co-Repressor Proteins/metabolism
- Gene Expression Regulation, Fungal
- Gene Expression Regulation, Plant
- Mediator Complex
- Mediator Complex/chemistry
- Mediator Complex/genetics
- Mediator Complex/metabolism
- Models, Molecular
- Nuclear Proteins
- Nuclear Proteins/genetics
- Nuclear Proteins/metabolism
- Plants, Genetically Modified
- Plants, Genetically Modified/genetics
- Plants, Genetically Modified/metabolism
- Protein Binding
- Protein Conformation, alpha-Helical
- Protein Interaction Domains and Motifs
- Repressor Proteins
- Repressor Proteins/genetics
- Repressor Proteins/metabolism
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae/genetics
- Saccharomyces cerevisiae/metabolism
- Saccharomyces cerevisiae Proteins
- Saccharomyces cerevisiae Proteins/genetics
- Saccharomyces cerevisiae Proteins/metabolism
- Structure-Activity Relationship