Budding yeast relies on G<sub>1</sub> cyclin specificity to couple cell cycle progression with morphogenetic development.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34088668.
- Also identified by DOI 10.1126/sciadv.abg0007 and PMC identifier 8177710.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Two models have been put forward for cyclin-dependent kinase (Cdk) control of the cell cycle. In the qualitative model, cell cycle events are ordered by distinct substrate specificities of successive cyclin waves. Alternatively, in the quantitative model, the gradual rise of Cdk activity from G<sub>1</sub> phase to mitosis leads to ordered substrate phosphorylation at sequential thresholds. Here, we study the relative contributions of qualitative and quantitative Cdk control in <i>Saccharomyces cerevisiae</i> All S phase and mitotic cyclins can be replaced by a single mitotic cyclin, albeit at the cost of reduced fitness. A single cyclin can also replace all G<sub>1</sub> cyclins to support ordered cell cycle progression, fulfilling key predictions of the quantitative model. However, single-cyclin cells fail to polarize or grow buds and thus cannot survive. Our results suggest that budding yeast has become dependent on G<sub>1</sub> cyclin specificity to couple cell cycle progression to essential morphogenetic events.