Nanopore sequencing of single-cell transcriptomes with scCOLOR-seq.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34211161.
- Also identified by DOI 10.1038/s41587-021-00965-w and PMC identifier 8668430.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Here we describe single-cell corrected long-read sequencing (scCOLOR-seq), which enables error correction of barcode and unique molecular identifier oligonucleotide sequences and permits standalone cDNA nanopore sequencing of single cells. Barcodes and unique molecular identifiers are synthesized using dimeric nucleotide building blocks that allow error detection. We illustrate the use of the method for evaluating barcode assignment accuracy, differential isoform usage in myeloma cell lines, and fusion transcript detection in a sarcoma cell line.
Medical subject headings
- Nanopore Sequencing