Single-nucleus RNA-seq2 reveals functional crosstalk between liver zonation and ploidy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34253736.
- Also identified by DOI 10.1038/s41467-021-24543-5 and PMC identifier 8275628.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Single-cell RNA-seq reveals the role of pathogenic cell populations in development and progression of chronic diseases. In order to expand our knowledge on cellular heterogeneity, we have developed a single-nucleus RNA-seq2 method tailored for the comprehensive analysis of the nuclear transcriptome from frozen tissues, allowing the dissection of all cell types present in the liver, regardless of cell size or cellular fragility. We use this approach to characterize the transcriptional profile of individual hepatocytes with different levels of ploidy, and have discovered that ploidy states are associated with different metabolic potential, and gene expression in tetraploid mononucleated hepatocytes is conditioned by their position within the hepatic lobule. Our work reveals a remarkable crosstalk between gene dosage and spatial distribution of hepatocytes.
Medical subject headings
- Liver
- Ploidies
- Sequence Analysis, RNA
- Single-Cell Analysis