Multiplexed direct detection of barcoded protein reporters on a nanopore array.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34385692.
- Also identified by DOI 10.1038/s41587-021-01002-6 and PMC identifier 8766897.
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Abstract
Detection of specific proteins using nanopores is currently challenging. To address this challenge, we developed a collection of over twenty nanopore-addressable protein tags engineered as reporters (NanoporeTERs, or NTERs). NTERs are constructed with a secretion tag, folded domain and a nanopore-targeting C-terminal tail in which arbitrary peptide barcodes can be encoded. We demonstrate simultaneous detection of up to nine NTERs expressed in bacterial or human cells using MinION nanopore sensor arrays.
Medical subject headings
- Nanopores