Advanced technologies for the preservation of mammalian biospecimens.

Huang, Haishui; He, Xiaoming; Yarmush, Martin L · Nat Biomed Eng · 2021

review · Level V

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Abstract

The three classical core technologies for the preservation of live mammalian biospecimens-slow freezing, vitrification and hypothermic storage-limit the biomedical applications of biospecimens. In this Review, we summarize the principles and procedures of these three technologies, highlight how their limitations are being addressed via the combination of microfabrication and nanofabrication, materials science and thermal-fluid engineering and discuss the remaining challenges.

Medical subject headings