Triplex Direct Quantitative Polymerase Chain Reaction for the Identification of Streptococcus pneumoniae Serotypes.
other · Level V
Where this comes from
- Record sourced from PubMed, PMID 34469558.
- Also identified by DOI 10.1093/infdis/jiab056 and PMC identifier 8414907.
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Abstract
The quantitative polymerase chain reaction (qPCR) method presented in this study allows the identification of pneumococcal capsular serotypes in cerebrospinal fluid without first performing DNA extraction. This testing approach, which saves time and resources, demonstrated similar sensitivity and a high level of agreement between cycle threshold values when it was compared side-by-side with the standard qPCR method with extracted DNA.
Medical subject headings
- Multiplex Polymerase Chain Reaction
- Pneumococcal Infections
- Streptococcus pneumoniae