Dissecting esophageal squamous-cell carcinoma ecosystem by single-cell transcriptomic analysis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34489433.
- Also identified by DOI 10.1038/s41467-021-25539-x and PMC identifier 8421382.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Esophageal squamous-cell carcinoma (ESCC), one of the most prevalent and lethal malignant disease, has a complex but unknown tumor ecosystem. Here, we investigate the composition of ESCC tumors based on 208,659 single-cell transcriptomes derived from 60 individuals. We identify 8 common expression programs from malignant epithelial cells and discover 42 cell types, including 26 immune cell and 16 nonimmune stromal cell subtypes in the tumor microenvironment (TME), and analyse the interactions between cancer cells and other cells and the interactions among different cell types in the TME. Moreover, we link the cancer cell transcriptomes to the somatic mutations and identify several markers significantly associated with patients' survival, which may be relevant to precision care of ESCC patients. These results reveal the immunosuppressive status in the ESCC TME and further our understanding of ESCC.
Medical subject headings
- Esophageal Neoplasms
- Esophageal Squamous Cell Carcinoma
- Neoplasm Proteins
- Stromal Cells
- Transcription, Genetic