Phosphobisaromatic motifs enable rapid enzymatic self-assembly and hydrogelation of short peptides.

Yi, Meihui; Guo, Jiaqi; He, Hongjian; Tan, Weiyi; Harmon, Nya; Ghebreyessus, Kesete; Xu, Bing · Soft Matter · 2021

basic_science · Level V

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Abstract

Enzyme-instructed self-assembly (EISA) and hydrogelation is a versatile approach for generating soft materials. Most of the substrates for alkaline phosphatase catalysed EISA utilize phosphotyrosine (<sub>p</sub>Tyr) as the enzymatic trigger for EISA and hydrogelation. Here we show the first example of phosphonaphthyl (<sub>p</sub>NP) and phosphobiphenyl (<sub>p</sub>BP) motifs acting as faster enzymatic triggers than phosphotyrosine for EISA and hydrogelation. This work illustrates novel enzyme triggers for rapid enzymatic self-assembly and hydrogelation.

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