Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles.

Ong, Sean Wei Xiang; Hon, Pei Yun; Wee, Sharon Syn Hui; Chia, Jonathan Wei Zhong; Mendis, Shehara; Izharuddin, Ezlyn; Lin, Ray Junhao; Chia, Po Ying et al. · Clin Infect Dis · 2022

cross_sectional · Level IV

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Abstract

We studied the performance of an algorithm combining multiplex polymerase chain reaction with phenotypic detection of extended-spectrum β-lactamases and carbapenemases directly from positive blood culture bottles in patients with gram-negative bacteremia and found good concordance with routine cultures. Such an algorithm may be a tool to improve time to optimal therapy in patients with gram-negative bacteremia.

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