Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34556671.
- Also identified by DOI 10.1038/s41467-021-25928-2 and PMC identifier 8460726.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Although prime editing is a promising genome editing method, the efficiency of prime editor 2 (PE2) is often insufficient. Here we generate a more efficient variant of PE2, named hyPE2, by adding the Rad51 DNA-binding domain. When tested at endogenous sites, hyPE2 shows a median of 1.5- or 1.4- fold (range, 0.99- to 2.6-fold) higher efficiencies than PE2; furthermore, at sites where PE2-induced prime editing is very inefficient (efficiency < 1%), hyPE2 enables prime editing with efficiencies ranging from 1.1% to 2.9% at up to 34% of target sequences, potentially facilitating prime editing applications.
Medical subject headings
- Algorithms
- CRISPR-Cas Systems
- DNA
- Gene Editing
- Models, Genetic
- Rad51 Recombinase