Ultrasensitive Detection of SARS-CoV-2 Antibody by Graphene Field-Effect Transistors.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34581586.
- Also identified by DOI 10.1021/acs.nanolett.1c00837.
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Abstract
The fast spread of SARS-CoV-2 has severely threatened the public health. Establishing a sensitive method for SARS-CoV-2 detection is of great significance to contain the worldwide pandemic. Here, we develop a graphene field-effect transistor (g-FET) biosensor and realize ultrasensitive SARS-CoV-2 antibody detection with a limit of detection (LoD) down to 10<sup>-18</sup> M (equivalent to 10<sup>-16</sup> g mL<sup>-1</sup>) level. The g-FETs are modified with spike S1 proteins, and the SARS-CoV-2 antibody biorecognition events occur in the vicinity of the graphene surface, yielding an LoD of ∼150 antibodies in 100 μL full serum, which is the lowest LoD value of antibody detection. The diagnoses time is down to 2 min for detecting clinical serum samples. As such, the g-FETs leverage rapid and precise SARS-CoV-2 screening and also hold great promise in prevention and control of other epidemic outbreaks in the future.
Medical subject headings
- Biosensing Techniques
- COVID-19
- Graphite