An adaptive optics module for deep tissue multiphoton imaging in vivo.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34608309.
- Also identified by DOI 10.1038/s41592-021-01279-0 and PMC identifier 9090585.
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Abstract
Understanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at great depths in vivo.
Medical subject headings
- Neuroimaging
- Optics and Photonics