Defining the Healthy Infant Metabolome: Liquid Chromatography Tandem-Mass Spectrometry Analysis of Dried Blood Spot Extracts from the Prospective Research on Early Determinants of Illness and Children's Health Trajectories Birth Cohort Study.

Schleif, William S; Harlan, Robert S; Hamblin, Frances; Amankwah, Ernest K; Goldenberg, Neil A; Hernandez, Raquel G; Johnson, Sara B; Reed, Shannon et al. · J Pediatr · 2022

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Abstract

Newborn screening using dried plasma spots offers preanalytical advantages over conventional cards for plasma-associated targets of interest. Herein we present dried plasma spot-based methods for measuring metabolites using a 250+ compound liquid chromatography tandem mass spectrometry library. Quality assurance reduced this library to 134, and from these, 30 compounds determined the normal newborn reference ranges.

Medical subject headings