Replication and single-cycle delivery of SARS-CoV-2 replicons.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34648371.
- Also identified by DOI 10.1126/science.abj8430 and PMC identifier 9007107.
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Abstract
Molecular virology tools are critical for basic studies of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and for developing new therapeutics. Experimental systems that do not rely on viruses capable of spread are needed for potential use in lower-containment settings. In this work, we use a yeast-based reverse genetics system to develop spike-deleted SARS-CoV-2 self-replicating RNAs. These noninfectious self-replicating RNAs, or replicons, can be trans-complemented with viral glycoproteins to generate replicon delivery particles for single-cycle delivery into a range of cell types. This SARS-CoV-2 replicon system represents a convenient and versatile platform for antiviral drug screening, neutralization assays, host factor validation, and viral variant characterization.
Medical subject headings
- RNA, Viral
- Replicon
- SARS-CoV-2