Fluidics system for resolving concentration-dependent effects of dissolved gases on tissue metabolism.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34734803.
- Also identified by DOI 10.7554/eLife.66716 and PMC identifier 8660022.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Oxygen (O<sub>2</sub>) and other dissolved gases such as the gasotransmitters H<sub>2</sub>S, CO, and NO affect cell metabolism and function. To evaluate effects of dissolved gases on processes in tissue, we developed a fluidics system that controls dissolved gases while simultaneously measuring parameters of electron transport, metabolism, and secretory function. We use pancreatic islets, retina, and liver from rodents to highlight its ability to assess effects of O<sub>2</sub> and H<sub>2</sub>S. Protocols aimed at emulating hypoxia-reperfusion conditions resolved a previously unrecognized transient spike in O<sub>2</sub> consumption rate (OCR) following replenishment of O<sub>2</sub>, and tissue-specific recovery of OCR following hypoxia. The system revealed both inhibitory and stimulatory effects of H<sub>2</sub>S on insulin secretion rate from isolated islets. The unique ability of this new system to quantify metabolic state and cell function in response to precise changes in dissolved gases provides a powerful platform for cell physiologists to study a wide range of disease states.
Medical subject headings
- Hydrogen Sulfide
- Islets of Langerhans
- Liver
- Oxygen
- Retina