Sensitive identification of neoantigens and cognate TCRs in human solid tumors.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34782741.
- Also identified by DOI 10.1038/s41587-021-01072-6 and PMC identifier 9110298.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The identification of patient-specific tumor antigens is complicated by the low frequency of T cells specific for each tumor antigen. Here we describe NeoScreen, a method that enables the sensitive identification of rare tumor (neo)antigens and of cognate T cell receptors (TCRs) expressed by tumor-infiltrating lymphocytes. T cells transduced with tumor antigen-specific TCRs identified by NeoScreen mediate regression of established tumors in patient-derived xenograft mice.
Medical subject headings
- Neoplasms
- Receptors, Antigen, T-Cell