Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34789723.
- Also identified by DOI 10.1038/s41467-021-26736-4 and PMC identifier 8599518.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Imaging the activity of neurons that are widely distributed across brain regions deep in scattering tissue at high speed remains challenging. Here, we introduce an open-source system with Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging (Diesel2p). Combining optical design, adaptive optics, and temporal multiplexing, the system offers subcellular resolution over a large field-of-view of ~25 mm<sup>2</sup>, encompassing distances up to 7 mm, with independent scan engines. We demonstrate the flexibility and various use cases of this system for calcium imaging of neurons in the living brain.
Medical subject headings
- Microscopy, Fluorescence, Multiphoton
- Nerve Net
- Optical Imaging