Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34789730.
- Also identified by DOI 10.1038/s41467-021-26737-3 and PMC identifier 8599611.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Understanding brain function requires monitoring local and global brain dynamics. Two-photon imaging of the brain across mesoscopic scales has presented trade-offs between imaging area and acquisition speed. We describe a flexible cellular resolution two-photon microscope capable of simultaneous video rate acquisition of four independently targetable brain regions spanning an approximate five-millimeter field of view. With this system, we demonstrate the ability to measure calcium activity across mouse sensorimotor cortex at behaviorally relevant timescales.
Medical subject headings
- Microscopy, Fluorescence, Multiphoton
- Neurons
- Optical Imaging