TDFragMapper: a visualization tool for evaluating experimental parameters in top-down proteomics.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34792554.
- Also identified by DOI 10.1093/bioinformatics/btab784 and PMC identifier 8796372.
- Licence recorded as CC BY-NC.
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Abstract
We present a new software-tool allowing an easy visualization of fragment ions and thus a rapid evaluation of key experimental parameters on the sequence coverage obtained for the MS/MS (tandem mass spectrometry) analysis of intact proteins. Our tool can process data obtained from various deconvolution and fragment assignment software. We demonstrate that TDFragMapper can rapidly highlight the experimental fragmentation parameters that are critical to the characterization of intact proteins of various size using top-down proteomics. TDFragMapper, a demonstration video and user tutorial are freely available for academic use at https://msbio.pasteur.fr/tdfragmapper; all data are thus available from the ProteomeXchange consortium (identifier PXD024643). Supplementary data are available at Bioinformatics online.
Medical subject headings
- Tandem Mass Spectrometry
- Proteomics