Single-cell analysis of diverse immune phenotypes in malignant pleural effusion.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34795282.
- Also identified by DOI 10.1038/s41467-021-27026-9 and PMC identifier 8602344.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The complex interactions among different immune cells have important functions in the development of malignant pleural effusion (MPE). Here we perform single-cell RNA sequencing on 62,382 cells from MPE patients induced by non-small cell lung cancer to describe the composition, lineage, and functional states of infiltrating immune cells in MPE. Immune cells in MPE display a number of transcriptional signatures enriched for regulatory T cells, B cells, macrophages, and dendritic cells compared to corresponding counterparts in blood. Helper T, cytotoxic T, regulatory T, and T follicular helper cells express multiple immune checkpoints or costimulatory molecules. Cell-cell interaction analysis identifies regulatory B cells with more interactions with CD4<sup>+</sup> T cells compared to CD8<sup>+</sup> T cells. Macrophages are transcriptionally heterogeneous and conform to M2 polarization characteristics. In addition, immune cells in MPE show the general up-regulation of glycolytic pathways associated with the hypoxic microenvironment. These findings show a detailed atlas of immune cells in human MPE and enhance the understanding of potential diagnostic and therapeutic targets in advanced non-small cell lung cancer.
Medical subject headings
- Gene Expression Profiling
- Gene Expression Regulation, Neoplastic
- Immunophenotyping
- Pleural Effusion, Malignant
- RNA-Seq
- Single-Cell Analysis