Organ-on-a-chip model of vascularized human bone marrow niches.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34810038.
- Also identified by DOI 10.1016/j.biomaterials.2021.121245 and PMC identifier 10658812.
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Abstract
Bone marrow niches (endosteal and perivascular) play important roles in both normal bone marrow function and pathological processes such as cancer cell dormancy. Unraveling the mechanisms underlying these events in humans has been severely limited by models that cannot dissect dynamic events at the niche level. Utilizing microfluidic and stem cell technologies, we present a 3D in vitro model of human bone marrow that contains both the perivascular and endosteal niches, complete with dynamic, perfusable vascular networks. We demonstrate that our model can replicate in vivo bone marrow function, including maintenance and differentiation of CD34<sup>+</sup> hematopoietic stem/progenitor cells, egress of neutrophils (CD66b<sup>+</sup>), and niche-specific responses to doxorubicin and granulocyte-colony stimulating factor. Our platform provides opportunities to accelerate current understanding of human bone marrow function and drug response with high spatial and temporal resolution.
Medical subject headings
- Bone Marrow
- Lab-On-A-Chip Devices