Mapping enzyme catalysis with metabolic biosensing.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34815408.
- Also identified by DOI 10.1038/s41467-021-27185-9 and PMC identifier 8611028.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Enzymes are represented across a vast space of protein sequences and structural forms and have activities that far exceed the best chemical catalysts; however, engineering them to have novel or enhanced activity is limited by technologies for sensing product formation. Here, we describe a general and scalable approach for characterizing enzyme activity that uses the metabolism of the host cell as a biosensor by which to infer product formation. Since different products consume different molecules in their synthesis, they perturb host metabolism in unique ways that can be measured by mass spectrometry. This provides a general way by which to sense product formation, to discover unexpected products and map the effects of mutagenesis.
Medical subject headings
- Biosensing Techniques
- Enzyme Assays
- Metabolic Engineering