MCMICRO: a scalable, modular image-processing pipeline for multiplexed tissue imaging.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34824477.
- Also identified by DOI 10.1038/s41592-021-01308-y and PMC identifier 8916956.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Highly multiplexed tissue imaging makes detailed molecular analysis of single cells possible in a preserved spatial context. However, reproducible analysis of large multichannel images poses a substantial computational challenge. Here, we describe a modular and open-source computational pipeline, MCMICRO, for performing the sequential steps needed to transform whole-slide images into single-cell data. We demonstrate the use of MCMICRO on tissue and tumor images acquired using multiple imaging platforms, thereby providing a solid foundation for the continued development of tissue imaging software.
Medical subject headings
- Image Processing, Computer-Assisted
- Neoplasms