A genetically encoded fluorescent biosensor for extracellular L-lactate.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34873165.
- Also identified by DOI 10.1038/s41467-021-27332-2 and PMC identifier 8648760.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
L-Lactate, traditionally considered a metabolic waste product, is increasingly recognized as an important intercellular energy currency in mammals. To enable investigations of the emerging roles of intercellular shuttling of L-lactate, we now report an intensiometric green fluorescent genetically encoded biosensor for extracellular L-lactate. This biosensor, designated eLACCO1.1, enables cellular resolution imaging of extracellular L-lactate in cultured mammalian cells and brain tissue.
Medical subject headings
- Bacterial Proteins
- Biosensing Techniques
- Green Fluorescent Proteins
- Lactic Acid
- Periplasmic Proteins
- Recombinant Fusion Proteins