Entropy-regularized deconvolution of cellular cryotransmission electron tomograms.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34876518.
- Also identified by DOI 10.1073/pnas.2108738118 and PMC identifier 8685678.
- Licence recorded as CC BY-NC-ND.
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Abstract
Cryo-electron tomography (cryo-ET) allows for the high-resolution visualization of biological macromolecules. However, the technique is limited by a low signal-to-noise ratio (SNR) and variance in contrast at different frequencies, as well as reduced Z resolution. Here, we applied entropy-regularized deconvolution (ER-DC) to cryo-ET data generated from transmission electron microscopy (TEM) and reconstructed using weighted back projection (WBP). We applied deconvolution to several in situ cryo-ET datasets and assessed the results by Fourier analysis and subtomogram analysis (STA).
Medical subject headings
- Cryoelectron Microscopy
- Entropy
- Saccharomyces cerevisiae