MLL4 mediates differentiation and tumor suppression through ferroptosis.

Egolf, Shaun; Zou, Jonathan; Anderson, Amy; Simpson, Cory L; Aubert, Yann; Prouty, Stephen; Ge, Kai; Seykora, John T et al. · Sci Adv · 2021

basic_science · Level V

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Abstract

The epigenetic regulator, <i>MLL4</i> (<i>KMT2D</i>), has been described as an essential gene in both humans and mice. In addition, it is one of the most commonly mutated genes in all of cancer biology. Here, we identify a critical role for Mll4 in the promotion of epidermal differentiation and ferroptosis, a key mechanism of tumor suppression. Mice lacking epidermal <i>Mll4</i>, but not the related enzyme <i>Mll3</i> (<i>Kmt2c</i>), display features of impaired differentiation and human precancerous neoplasms, all of which progress with age. Mll4 deficiency profoundly alters epidermal gene expression and uniquely rewires the expression of key genes and markers of ferroptosis (<i>Alox12</i>, <i>Alox12b</i>, and <i>Aloxe3</i>). Beyond revealing a new mechanistic basis for <i>Mll4</i>-mediated tumor suppression, our data uncover a potentially much broader and general role for ferroptosis in the process of differentiation and skin homeostasis.