Simultaneous two-photon imaging of action potentials and subthreshold inputs in vivo.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34893595.
- Also identified by DOI 10.1038/s41467-021-27444-9 and PMC identifier 8664861.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
To better understand the input-output computations of neuronal populations, we developed ArcLight-ST, a genetically-encoded voltage indicator, to specifically measure subthreshold membrane potentials. We combined two-photon imaging of voltage and calcium, and successfully discriminated subthreshold inputs and spikes with cellular resolution in vivo. We demonstrate the utility of the method by mapping epileptic seizures progression through cortical circuits, revealing divergent sub- and suprathreshold dynamics within compartmentalized epileptic micronetworks. Two-photon, two-color imaging of calcium and voltage enables mapping of inputs and outputs in neuronal populations in living animals.
Medical subject headings
- Action Potentials
- Membrane Potentials
- Neurons
- Optical Imaging
- Photons