Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34914768.
- Also identified by DOI 10.1371/journal.pone.0261417 and PMC identifier 8675714.
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Abstract
The use of clearing agents has provided new insights in various fields of medical research (developmental biology, neurology) by enabling examination of tissue architecture in 3D. One of the challenges is that clearing agents induce tissue shrinkage and the shrinkage rates reported in the literature are incoherent. Here, we report that for a classical clearing agent, benzyl-alcohol benzyl-benzoate (BABB), the shrinkage decreases significantly with increasing sample size, and present an analytical formula describing this.
Medical subject headings
- Benzoates
- Benzyl Alcohol
- Imaging, Three-Dimensional
- Optical Imaging
- Solvents