Transcriptional network orchestrating regional patterning of cortical progenitors.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34921112.
- Also identified by DOI 10.1073/pnas.2024795118 and PMC identifier 8713794.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We uncovered a transcription factor (TF) network that regulates cortical regional patterning in radial glial stem cells. Screening the expression of hundreds of TFs in the developing mouse cortex identified 38 TFs that are expressed in gradients in the ventricular zone (VZ). We tested whether their cortical expression was altered in mutant mice with known patterning defects (<i>Emx2, Nr2f1</i>, and <i>Pax6</i>), which enabled us to define a cortical regionalization TF network (CRTFN). To identify genomic programming underlying this network, we performed TF ChIP-seq and chromatin-looping conformation to identify enhancer-gene interactions. To map enhancers involved in regional patterning of cortical progenitors, we performed assays for epigenomic marks and DNA accessibility in VZ cells purified from wild-type and patterning mutant mice. This integrated approach has identified a CRTFN and VZ enhancers involved in cortical regional patterning in the mouse.
Medical subject headings
- Cerebral Cortex
- Gene Regulatory Networks
- Regulatory Elements, Transcriptional
- Transcription Factors