Pairs and Pairix: a file format and a tool for efficient storage and retrieval for Hi-C read pairs.

Lee, Soohyun; Bakker, Clara R; Vitzthum, Carl; Alver, Burak H; Park, Peter J · Bioinformatics · 2022

basic_science · Level V

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Abstract

As the amount of 3D chromosomal interaction data continues to increase, storing and accessing such data efficiently becomes paramount. We introduce Pairs, a block-compressed text file format for storing paired genomic coordinates from Hi-C data, and Pairix, an open-source C application to index and query Pairs files. Pairix (also available in Python and R) extends the functionalities of Tabix to paired coordinates data. We have also developed PairsQC, a collapsible HTML quality control report generator for Pairs files. The format specification and source code are available at https://github.com/4dn-dcic/pairix, https://github.com/4dn-dcic/Rpairix and https://github.com/4dn-dcic/pairsqc.

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