Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 35027766.
- Also identified by DOI 10.1038/s41592-021-01356-4 and PMC identifier 8828483.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Cryofixation has proven to be the gold standard for efficient preservation of native cell ultrastructure compared to chemical fixation, but this approach is not widely used in fluorescence microscopy owing to implementation challenges. Here, we develop Cryo-ExM, a method that preserves native cellular organization by coupling cryofixation with expansion microscopy. This method bypasses artifacts associated with chemical fixation and its simplicity will contribute to its widespread use in super-resolution microscopy.
Medical subject headings
- Cryopreservation
- Microscopy, Fluorescence