Lassa Virus Replicon Particle Vaccine Protects Strain 13/N Guinea Pigs Against Challenge With Geographically and Genetically Diverse Viral Strains.

Spengler, Jessica R; Kainulainen, Markus H; Welch, Stephen R; Coleman-McCray, JoAnn D; Harmon, Jessica R; Condrey, Jillian A; Scholte, Florine E M; Nichol, Stuart T et al. · J Infect Dis · 2022

basic_science · Level V

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Abstract

Lassa virus (LASV) causes mild to severe hemorrhagic fever disease in humans. Strain 13/N guinea pigs are highly susceptible to infection with LASV strain Josiah (clade IV), providing a critical model system for therapeutics and vaccine development. To develop additional models of disease, we detail the clinical course in guinea pigs infected with 5 geographically and genetically diverse LASV strains. Two of the developed models (LASV clades II and III) were then used to evaluate efficacy of a virus replicon particle vaccine against heterologous LASV challenge, demonstrating complete protection against clinical disease after a single vaccination dose.

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