Rapid cell pairing and fusion based on oscillating bubbles within an acoustofluidic device.

Abstract

Cell fusion is an essential event in many biological processes and has gained increasing attention in the field of biotechnology. In this study, we demonstrate an effective and convenient strategy for cell capture, pairing, and fusion based on oscillating bubbles within an acoustofluidic device. Multirectangular structures of the same size were fabricated at the sidewall of polydimethylsiloxane to generate monodisperse microbubbles. These microbubbles oscillated with a similar amplitude under single-frequency acoustic excitation. Cells were simultaneously captured and paired on the surface of the oscillating bubbles within 40 ms, and the efficiency reached approximately 90%. Homotypic or heterotypic cell membrane fusion was achieved within 15 and 20 min, respectively. More importantly, the homotypic fused cells enabled migration and proliferation at 24 h, indicating that the important biological functions were not altered.