Loss of MIG-6 results in endometrial progesterone resistance via ERBB2.

Yoo, Jung-Yoon; Kim, Tae Hoon; Shin, Jung-Ho; Marquardt, Ryan M; Müller, Ulrich; Fazleabas, Asgerally T; Young, Steven L; Lessey, Bruce A et al. · Nat Commun · 2022

basic_science · Level V

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Abstract

Female subfertility is highly associated with endometriosis. Endometrial progesterone resistance is suggested as a crucial element in the development of endometrial diseases. We report that MIG-6 is downregulated in the endometrium of infertile women with endometriosis and in a non-human primate model of endometriosis. We find ERBB2 overexpression in the endometrium of uterine-specific Mig-6 knockout mice (Pgr<sup>cre/+</sup>Mig-6<sup>f/f</sup>; Mig-6<sup>d/d</sup>). To investigate the effect of ERBB2 targeting on endometrial progesterone resistance, fertility, and endometriosis, we introduce Erbb2 ablation in Mig-6<sup>d/d</sup> mice (Mig-6<sup>d/d</sup>Erbb2<sup>d/d</sup> mice). The additional knockout of Erbb2 rescues all phenotypes seen in Mig-6<sup>d/d</sup> mice. Transcriptomic analysis shows that genes differentially expressed in Mig-6<sup>d/d</sup> mice revert to their normal expression in Mig-6<sup>d/d</sup>Erbb2<sup>d/d</sup> mice. Together, our results demonstrate that ERBB2 overexpression in endometrium with MIG-6 deficiency causes endometrial progesterone resistance and a nonreceptive endometrium in endometriosis-related infertility, and ERBB2 targeting reverses these effects.

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