The SpliZ generalizes 'percent spliced in' to reveal regulated splicing at single-cell resolution.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 35241832.
- Also identified by DOI 10.1038/s41592-022-01400-x and PMC identifier 9089759.
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Abstract
Detecting single-cell-regulated splicing from droplet-based technologies is challenging. Here, we introduce the splicing Z score (SpliZ), an annotation-free statistical method to detect regulated splicing in single-cell RNA sequencing. We applied the SpliZ to human lung cells, discovering hundreds of genes with cell-type-specific splicing patterns including ones with potential implications for basic and translational biology.
Medical subject headings
- Alternative Splicing
- RNA Splicing