Thermodynamically coupled biosensors for detecting neutralizing antibodies against SARS-CoV-2 variants.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 35484405.
- Also identified by DOI 10.1038/s41587-022-01280-8 and PMC identifier 9463068.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We designed a protein biosensor that uses thermodynamic coupling for sensitive and rapid detection of neutralizing antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants in serum. The biosensor is a switchable, caged luciferase-receptor-binding domain (RBD) construct that detects serum-antibody interference with the binding of virus RBD to angiotensin-converting enzyme 2 (ACE-2) as a proxy for neutralization. Our coupling approach does not require target modification and can better distinguish sample-to-sample differences in analyte binding affinity and abundance than traditional competition-based assays.
Medical subject headings
- Biosensing Techniques
- COVID-19