Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 35637419.
- Also identified by DOI 10.1038/s41587-022-01312-3 and PMC identifier 9546769.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be easily miniaturized to decrease resource consumption. The FS protocol can also use unique molecular identifiers (UMIs) for molecule counting while displaying reduced strand-invasion artifacts. FS will be especially useful for characterizing gene expression at high resolution across multiple samples.
Medical subject headings
- RNA
- Single-Cell Analysis