Ethylene modulates translation dynamics in <i>Arabidopsis</i> under submergence via GCN2 and EIN2.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 35658031.
- Also identified by DOI 10.1126/sciadv.abm7863 and PMC identifier 9166634.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
General translational repression is a key process that reduces energy consumption under hypoxia. Here, we show that plant stress-activated general control nonderepressible 2 (GCN2) was activated to regulate the reduction in polysome loading during submergence in <i>Arabidopsis</i>. GCN2 signaling was activated by ethylene under submergence. GCN2 activity was reduced in <i>etr1-1</i>, but not in <i>ein2-5</i> or <i>eil1ein3</i>, under submergence, suggesting that GCN2 activity is regulated by a noncanonical ethylene signaling pathway. Polysome loading was not reduced in <i>ein2-5</i> under submergence, implying that ethylene modulates translation via both EIN2 and GCN2. Transcriptomic analysis demonstrated that EIN2 and GCN2 regulate not only general translational repression but also translational enhancement of specific mRNAs under submergence. Together, these results demonstrate that during submergence, entrapped ethylene triggers GCN2 and EIN2 to regulate translation dynamics and ensure the translation of stress response proteins.