Global fitting for high-accuracy multi-channel single-molecule localization.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 35668089.
- Also identified by DOI 10.1038/s41467-022-30719-4 and PMC identifier 9170706.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Multi-channel detection in single-molecule localization microscopy greatly increases information content for various biological applications. Here, we present globLoc, a graphics processing unit based global fitting algorithm with flexible PSF modeling and parameter sharing, to extract maximum information from multi-channel single molecule data. As signals in multi-channel data are highly correlated, globLoc links parameters such as 3D coordinates or photon counts across channels, improving localization precision and robustness. We show, both in simulations and experiments, that global fitting can substantially improve the 3D localization precision for biplane and 4Pi single-molecule localization microscopy and color assignment for ratiometric multicolor imaging.
Medical subject headings
- Nanotechnology
- Single Molecule Imaging