The Impact of Peptide Amount on Tumor Uptake to Assess PSMA Receptor Saturation on ⁶⁸Ga-PSMA-11 PET/CT in Patients with Primary Prostate Cancer.

Abstract

⁶⁸Ga-labeled prostate-specific membrane antigen (PSMA) is often produced on-site, where usually a fixed amount of peptide is conjugated to the generator eluate. However, fluctuations in specific activity might influence tracer distribution and tumor accumulation. Therefore, our aim was to investigate the potential effect of varying the administered peptide amount on ⁶⁸Ga-PSMA-11 uptake in tumors using PET/CT in patients with primary prostate cancer (PCa). Additionally, the impact of tumor volume on this potential effect and on accumulation in reference organs was assessed. <b>Methods:</b> The imaging data of 362 men with primary PCa who underwent ⁶⁸Ga-PSMA-11 PET/CT were retrospectively included. Scans were quantified for normal tissue and primary tumors. Patients were divided into 3 groups based on their tumor volume. Correlation and multivariable linear regression analyses were performed. <b>Results:</b> The median index lesion volume was 9.50 cm³ (range, 0.064-174 cm³). Groups were based on quartiles of prostatic lesion volume: ≤4.11 cm³ (group 1), 4.11-20.6 cm³ (group 2), and ≥20.6 cm³ (group 3). No correlation was found between administered peptide amount and tumor uptake (SUV_mean or SUV_peak) for any group, except for a significant correlation for SUV_mean in the first group (<i>P</i> = 0.008). Linear regression analysis supported these findings. <b>Conclusion:</b> The amount of administered peptide had no evident effect on ⁶⁸Ga-PSMA-11 uptake in tumors, except for a significant positive correlation between administered peptide amount and tumor SUV_mean for group 1. The findings imply that no receptor saturation occurs in men with primary PCa at peptide levels of about 2.5 μg.