A long noncoding RNA promotes parasite differentiation in African trypanosomes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 35704590.
- Also identified by DOI 10.1126/sciadv.abn2706 and PMC identifier 9200285.
- Licence recorded as CC BY-NC.
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Abstract
The parasite <i>Trypanosoma brucei</i> causes African sleeping sickness that is fatal to patients if untreated. Parasite differentiation from a replicative slender form into a quiescent stumpy form promotes host survival and parasite transmission. Long noncoding RNAs (lncRNAs) are known to regulate cell differentiation in other eukaryotes. To determine whether lncRNAs are also involved in parasite differentiation, we used RNA sequencing to survey the <i>T. brucei</i> genome, identifying 1428 previously uncharacterized lncRNA genes. We find that <i>grumpy</i> lncRNA is a key regulator that promotes parasite differentiation into the quiescent stumpy form. This function is promoted by a small nucleolar RNA encoded within the <i>grumpy</i> lncRNA. <i>snoGRUMPY</i> binds to messenger RNAs of at least two stumpy regulatory genes, promoting their expression. <i>grumpy</i> overexpression reduces parasitemia in infected mice. Our analyses suggest that <i>T. brucei</i> lncRNAs modulate parasite-host interactions and provide a mechanism by which <i>grumpy</i> regulates cell differentiation in trypanosomes.