Measuring SARS-CoV-2 T cell immunity with a scalable qPCR-based assay.

Nat Biotechnol · 2022

basic_science · Level V

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Abstract

We developed two quantitative PCR-based assays to detect SARS-CoV-2-specific T cell immunity: qTACT and dqTACT. The assays quantify <i>CXCL10</i> mRNA, after incubation of whole blood with viral peptides, as a proxy of an antigen-specific T cell response, and will allow population-level monitoring of cellular immunity to SARS-CoV-2.

Medical subject headings