Peptide fusion improves prime editing efficiency.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 35717416.
- Also identified by DOI 10.1038/s41467-022-31270-y and PMC identifier 9206660.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Prime editing enables search-and-replace genome editing but is limited by low editing efficiency. We present a high-throughput approach, the Peptide Self-Editing sequencing assay (PepSEq), to measure how fusion of 12,000 85-amino acid peptides influences prime editing efficiency. We show that peptide fusion can enhance prime editing, prime-enhancing peptides combine productively, and a top dual peptide-prime editor increases prime editing significantly in multiple cell lines across dozens of target sites. Top prime-enhancing peptides function by increasing translation efficiency and serve as broadly useful tools to improve prime editing efficiency.
Medical subject headings
- CRISPR-Cas Systems
- Gene Editing