Cenozoic megatooth sharks occupied extremely high trophic positions.

Kast, Emma R; Griffiths, Michael L; Kim, Sora L; Rao, Zixuan C; Shimada, Kenshu; Becker, Martin A; Maisch, Harry M; Eagle, Robert A et al. · Sci Adv · 2022

basic_science · Level V

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Abstract

Trophic position is a fundamental characteristic of animals, yet it is unknown in many extinct species. In this study, we ground-truth the <sup>15</sup>N/<sup>14</sup>N ratio of enameloid-bound organic matter (δ<sup>15</sup>N<sub>EB</sub>) as a trophic level proxy by comparison to dentin collagen δ<sup>15</sup>N and apply this method to the fossil record to reconstruct the trophic level of the megatooth sharks (genus <i>Otodus</i>). These sharks evolved in the Cenozoic, culminating in <i>Otodus megalodon</i>, a shark with a maximum body size of more than 15 m, which went extinct 3.5 million years ago. Very high δ<sup>15</sup>N<sub>EB</sub> values (22.9 ± 4.4‰) of <i>O. megalodon</i> from the Miocene and Pliocene show that it occupied a higher trophic level than is known for any marine species, extinct or extant. δ<sup>15</sup>N<sub>EB</sub> also indicates a dietary shift in sharks of the megatooth lineage as they evolved toward the gigantic <i>O. megalodon</i>, with the highest trophic level apparently reached earlier than peak size.