Liquid exfoliation of V₈C₇ nanodots as peroxidase-like nanozymes for photothermal-catalytic synergistic antibacterial treatment.

Abstract

Nanozymes are effective antibiotics that use reactive oxygen species (ROS) produced by Fenton/Fenton-like reactions to kill bacteria. However, its activity is still not satisfactory and requires large amounts of hydrogen peroxide (H₂O₂) with side effects on normal tissues. Herein, ultrasmall V₈C₇ nanodots (NDs) are successfully constructed by the liquid-phase exfoliation method for photothermal-catalytic synergistic antibacterial treatment. The prepared V₈C₇ NDs are horseradish peroxidase (HRP)-like nanozymes that can efficiently catalyze H₂O₂ to produce a large amount of ROS. Unlike traditional HRP-like nanozymes, V₈C₇ NDs can have a good catalytic effect under slightly acidic conditions (pH=5.5). Moreover, V₈C₇ NDs have good near-infrared (NIR) absorption and high photothermal conversion efficiency (PTCE, 50.39%), which can be used for photothermal treatment (PTT) of bacteria. In addition, the mild photothermal effect can further enhance the HRP-like catalytic activity of V₈C₇ NDs, thereby further enhancing the antibacterial performance of V₈C₇ NDs. In vitro results show that V₈C₇ NDs can effectively eradicate Escherichia coli (E. coli, gram-negative) and methicillin-resistant Staphylococcus aureus (MRSA, gram-positive) under laser irradiation and the presence of H₂O₂, possessing spectral antibacterial properties. More importantly, V₈C₇ NDs display satisfactory therapeutic effects on wounds infected by MRSA in vivo, and their toxicity is negligible, suggesting that they may have great potential for application as powerful and safe antibacterial agents. This work presents a practical antibacterial strategy by combining PTT and catalytic therapy to achieve efficient treatment of bacteria-infected wounds. STATEMENT OF SIGNIFICANCE: (1) Ultrasmall V₈C₇ NDs were prepared by the liquid-phase exfoliation method. (2) V₈C₇ NDs showed good photothermal, catalytic properties. (3) V₈C₇ NDs achieved satisfactory photothermal-catalytic synergistic antibacterial treatment.