Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 35817767.
- Also identified by DOI 10.1038/s41467-022-31628-2 and PMC identifier 9273615.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Auxin critically regulates plant growth and development. Auxin-driven transcriptional responses are mediated through the AUXIN RESPONSE FACTOR (ARF) family of transcription factors. ARF protein condensation attenuates ARF activity, resulting in dramatic shifts in the auxin transcriptional landscape. Here, we perform a forward genetics screen for ARF hypercondensation, identifying an F-box protein, which we named AUXIN RESPONSE FACTOR F-BOX1 (AFF1). Functional characterization of SCF<sup>AFF1</sup> revealed that this E3 ubiquitin ligase directly interacts with ARF19 and ARF7 to regulate their accumulation, condensation, and nucleo-cytoplasmic partitioning. Mutants defective in AFF1 display attenuated auxin responsiveness, and developmental defects, suggesting that SCF<sup>AFF1</sup> -mediated regulation of ARF protein drives aspects of auxin response and plant development.
Medical subject headings
- Arabidopsis
- Arabidopsis Proteins