Overexpression of the scopoletin biosynthetic pathway enhances lignocellulosic biomass processing.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 35857515.
- Also identified by DOI 10.1126/sciadv.abo5738 and PMC identifier 9278857.
- Licence recorded as CC BY-NC.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Lignin is the main factor limiting the enzymatic conversion of lignocellulosic biomass into fermentable sugars. To reduce the recalcitrance engendered by the lignin polymer, the coumarin scopoletin was incorporated into the lignin polymer through the simultaneous expression of <i>FERULOYL-CoA 6'-HYDROXYLASE 1</i> (<i>F6'H1</i>) and <i>COUMARIN SYNTHASE</i> (<i>COSY</i>) in lignifying cells in <i>Arabidopsis</i>. The transgenic lines overproduced scopoletin and incorporated it into the lignin polymer, without adversely affecting plant growth. About 3.3% of the lignin units in the transgenic lines were derived from scopoletin, thereby exceeding the levels of the traditional <i>p-</i>hydroxyphenyl units. Saccharification efficiency of alkali-pretreated scopoletin-overproducing lines was 40% higher than for wild type.